Silage as a raw material for the chemical industry

(German title: Silage als Rohstoff für die chemische Industrie)

Grass is a globally widespread renewable raw material with high biomass yields. For industrial use, however, the problem of storing grass cuttings must be taken into account. Ensiling is a good way of making grass cuttings suitable for long-term storage. The lactic acid fermentation that takes place during this process leads to the preservation of the grass cuttings on the one hand and on the other hand already results in an initial increase in value, as lactic acid is one of the most important platform chemicals for plastics made from renewable raw materials.

In a joint project between the Department of Bioprocess Engineering and the Department of Thermal Process Engineering at the Technical University of Kaiserslautern and the Technical Biochemistry working group at Saarland University, the use of grass silage as a raw material for fermentative and chemical-catalytic processes for the production of basic and fine chemicals is to be developed. On the one hand, the extent to which silage can be fermented directly and, on the other hand, how fermentation characteristics can be improved by simple processing methods will be investigated. In a first process step, a pressed juice is obtained from the silage, from which a raw lactic acid fraction is obtained using extractive processes. Secondly, the hydrolysis of the polysaccharides contained in the silage is investigated. These raw fractions are then used in fermentative processes to produce solvents (ethanol, 1,2-propanediol) and basic chemicals (itaconic acid, succinic acid) or amino acids. The products produced are to be obtained as in-situ as possible using innovative processes. The silage should be used as completely as possible. This is why, for example, the raffinate from lactic acid extraction is used as a supplement in the fermentations. In addition, all residues are to be used for the production of biogas.

Partner

Lehrgebiet Thermische Verfahrenstechnik (TU Kaiserslautern), Prof. Hans-Jörg Bart
Technische Biochemie (Universität des Saarlandes), Prof. Elmar Heinzle

StatusCompleted project
Funding organisationBMELV/FNR
Funding period06/2008-05/2011
Funding code22025407

Publications and conference papers

  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Silage -Fermentationsrohstoff für die chemische Industrie? Labor & More 2/09, S. 44-45
  • T. Sieker, A. Neuner, D. Dimitrova, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Verfahrensentwicklung für die Nutzung von Gras-Silage als Rohstoff für die chemische Industrie. Chemie Ingenieur Technik 82 (8) 1153-1159
  • A. Neuner, I. Wagner, R. Ulber, K. Schneider, S. Peifer, E. Heinzle; Production of l-lysine on different silage juices using genetically engineered Corynebacterium glutamicum; J. Biotechnol. 163 (2013) 217– 224
  • T. Sieker, R. Ulber; First Steps in the Process Development for Ethanol Production from Grass Silage by Simultaneous Pretreatment, Saccharification and Fermentation; Engineering in Life Sciences; Eng. Life Sci. 4 (2011) 436–442
  • T. Sieker, R. Ulber; Production of bulk and fine chemicals using silage as a renewable resource. 2nd Workshop on Fats and Oils as Renewable Feedstock for the Chemical Industry (2009)
  • T. Sieker, N. Tippkötter, R. Ulber; Silage as feedstock for the chemical industry. Messe ACHEMA, Frankfurt am Main (2009)
  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Produktion von Grundchemikalien aus Silage. DECHEMA Vortrags- und Diskussionstagung Biokatalyse: Neue Verfahren, neue Produkte, (2009)
  • R. Ulber, T. Sieker; Plattformchemikalien aus Silage-Säften; Deutscher Bioraffineriekongress (2009) Potsdam
  • T. Sieker, D. Dimitrova, N. Neuner, N. Tippkötter, H.J. Bart, E. Heinzle, R. Ulber; Utilization of silage as fermentation substrate for the chemical industry. RRB5, (2009)
  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Nutzung von Silage zur fermentativen Produktion von Grund- und Feinchemikalien. 27. Jahrestagung der Biotechnologen (2009)
  • A. Neuner, E. Heinzle; Metabolic engineering of Corynebacterium glutamicum for Lysine Production from Silage: Lactate Conversion to Lysine. 27. Jahrestagung der Biotechnologen (2009)
  • R. Ulber; Nutzung von Silage als Rohstoff für die industrielle (weiße) Biotechnologie; DECHEMA-Tagung Industrielle Nutzung nachwachsender Rohstoffe - Chemie, Biotechnologie, Verfahrenstechnik (2010) Frankfurt
  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, E. Heinzle, H.-J. Bart, R. Ulber; An integrated process for the simultaneous enzymatic pretreatment, enzymatic hydrolysis and fermentation of grass silage lignocelluloses. 43. Jahrestreffen deutscher Katalytiker (2010) Weimar
  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Prozessintegration in der grünen Bioraffinerie: Das Simultanious pretreatment, saccharification and fermentation-Verfahren. DECHEMA Vortrags- und Diskussionstagung Bioprozessorientiertes Anlagendesign (2010) Nürnberg
  • T. Sieker, N. Tippkötter, R. Ulber; Integration of Process Steps in the Green Biorefinery by Simultaneous Pretreatment, Saccharification and Fermentation. European Workshop on Lignocellulosics and Pulp, Hamburg (2010)
  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Grüne Bioraffinerie: Ganzheitliche Nutzung von Grassilage für die Herstellung von Grund- und Feinchemikalien. 28. Jahrestagung der Biotechnologen / ProcessNet (2010) Aachen
  • T. Sieker, A. Neuner, D. Dimitrova, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber; Simultane Vorbehandlung, Hydrolyse und Fermentation bei der Nutzung von grüner Biomasse zur Produktion von Bioethanol. 28. Jahrestagung der Biotechnologen / ProcessNet (2010) Aachen
  • Sebastian Poth, Tim Sieker, Roland Ulber; Nutzung von Presssäften nachwachsender Rohstoffe. 28. Jahrestagung der Biotechnologen / ProcessNet (2010) Aachen
  • T. Sieker, D. Dimitrova, A. Neuner, N. Tippkötter, K. Muffler, H.-J. Bart, E. Heinzle, R. Ulber; A Green Biorefinery for the Production of Ethanol, Organic Acids and Polyphenols from Grass Silage. 1st European Congress on Applied Biotechnoloy (2011) Berlin
  • T. Sieker, A. Duwe, K. Muffler, N. Tippkötter, R. Ulber; A Silage-based Circular Flow Green Biorefinery. Industrial Use of Renewable Resources (2012) Frankfurt
  • T. Sieker, N. Tippkötter, K. Muffler, R. Ulber; Eine grüne Bioraffinerie zur Gewinnung von organischen Säuren, Phenolsäuren und Ethanol. Vortrags- und Diskussionstagung Biopharmazeutische Produktion (2012) Freiburg
  • T. Sieker, A. Duwe, D. Dimitrova, A. Neuner, K. Muffler, N. Tippkötter, H.-J. Bart, E. Heinzle, R. Ulber, Production of Organic and Phenolic Acids in a Circular Flow Green Biorefinery. 8th Conference on Renewable Resources and Biorefineries (2012) Toulouse
  • T. Sieker, A. Duwe, N. Tippkötter, K. Muffler, R. Ulber: Herstellung von Ethanol, Phenolsäuren, organischen Säuren und Biogas durch vollständige Nutzung von Grassilage in einer Bioraffinerie. 30. Jahrestagung der Biotechnologen (2012) Karlsruhe