Publikation: In-vivo and in-silico screening for antimicrobial compounds from cyanobacteria

Das Lehrgebiet veröffentlicht einen Übersichtsartikel in der Zeitschrift "Microbiology Open" unter dem Titel "In-vivo and in-silico screening for antimicrobial compounds from cyanobacteria".

Due to the emerging rise of multi-drug resistant bacteria, the discovery of novel antibiotics is of high scientific interest. Through their high chemodiversity of bioactive secondary metabolites, cyanobacteria have proven to be promising microorganisms for the discovery of antibacterial compounds. These aspects make appropriate antibacterial screening approaches for cyanobacteria crucial. Up to date, screenings are mostly carried out using phenotypical methodology, consisting of cyanobacterial cultivation, extraction and inhibitory assays. However, parameters of these methods highly vary within literature. Therefore, the common choices of parameters and inhibitory assays are summarized in this review. Nevertheless, less frequently used method variants are highlighted, which lead to hits from antimicrobial compounds. In addition to the considerations of phenotypic methods, this review provides an overview of developments in the genome-based screening area, be it in-vivo using PCR technique or in-silico using recent genome-mining method. Though, up to date these techniques are a lot less applied than phenotypical screening.

D. Strieth, S. Lenz, R. Ulber; In-vivo and in-silico screening for antimicrobial compounds from cyanobacteria, Microbiology Open, 2022, 10.1002/mbo3.1268