Das Lehrgebiet BioVT publiziert mit Partnern von der Tanta University (Ägypten), der Menoufia University (Ägypten), dem Center for Plant Systems Biology and Biotechnology Plovdiv (Bulgarien) und dem Max-Planck-Institut für Molekulare Pflanzenphysiologie (Potsdam) den Artikel "Time-dependent behaviour of methyl jasmonate elicited cell suspension cultures of Ocimum species via untargeted mass spectrometry-based metabolomics" in der Zeitschrift Industrial Crops & Products.
Ocimum (basil) is a widespread genus commonly consumed for culinary and medicinal purposes attributed to unique secondary metabolites. The present research investigated the metabolic profiles of two Ocimum suspension cultures derived from O. basilicum and O. americanum, following elicitation by methyl jasmonate at different time intervals (24, 48, and 72 h) compared to the baseline at T= 0 h identifying bioactive metabolites and the influence on their expressional behaviour. Based on comprehensive extraction and mass spectrometry (MS)-based analytical protocols, the results showed that phenolic acid abundances, particularly rosmarinic acid, were increased significantly in O. americanum culture after 72 h to a 2.0-fold increase (p<0.05). Similarly, its 3-O-methyl derivative was increased by 1.6-fold after 48 h in O. basilicum, and drastically (21.9-fold) after 72 h in O. americanum. Moreover, 23 out of the 56 identified bioactive secondary metabolites showed variable importance in the projection (VIP) scores ≥1, including isovitexin and 2,3-dihydroxybenzoic acid for O. basilicum. Furthermore, analysis of primary metabolites indicated that aromatic amino acids and sugars decreased indicating higher rates of consumption, while organic acids as end products of metabolisms increased significantly following elicitation for O. basilicum. Besides, the lipid profile showed a total of 107 metabolites. However, the effect of elicitation was variable between both species. Hence, the integration of untargeted metabolomics with chemical elicitation may improve our knowledge regarding the effects on various metabolites accumulation behaviour of different basil suspension cultures in a comparative and time-dependent approach.
A. Mehring, A. Zayed, M.A. Salem, S. Alseekh, A.R. Fernie, R. Ulber; Time-dependent behaviour of methyl jasmonate elicited cell suspension cultures of Ocimum species via untargeted mass spectrometry-based metabolomics; Industrial Crops & Products (2022) im Druck