Production of bioactive compounds by brown algea cell cultures
This research project aims to overcome the major problems of seaweed farming and industrial-scale cultivation by development of closed-system bioreactors for the culture of the seaweeds for the production of bioactive compounds such as fucoidans. Fucus vesiculosus will be taken as an example of brown algae. The research project will be divided into two parts.
Part 1:
Before starting the production process, many points should be optimized on the dried powder to be applied later. For example:
- Optimization of fucoidan extraction,
- Optimization of fucoidan-assay and fucoidan-analytics,
- Investigation of the anticoagulant activity, and
- Structure elucidation.
Part 2:
The tissue culture part will be focused on:
- Explant sterilization,
- Effect of sterilization protocol on the vitality,
- Growth of the explant on different solid media and study the effect of different temperature, pH, light and hormones,
- Growth in suspension culture, and
- Investigation of the growth and production in wave bag bioreactor.
| Partner | - |
| Status | Completed project |
| Funding organization | DAAD |
| Funding period | 04/2014 - 06/2018 |
| Funding code | PhD fund |
| Employee |
Publications and conference papers
- A. Zayed, T. Hahn, D. Finkelmeier, A. Burger-Kentischer, S. Rupp, R. Krämer, R. Ulber; Phenomenological Investigation of the Cytotoxic Activity of Fucoidan Isolated from Fucus vesiculosus; Process Biochemistry (2019) https://doi.org/10.1016/j.procbio.2019.03.026
A. Zayed, R. Ulber, Fucoidan Production: Approval Key Challenges and Opportunities, Carbohydrate Polymers (2019), https://doi.org/10.1016/j.carbpol.2019.01.10
A. Zayed, M. Kovacheva, K. Muffler, H.-W. Breiner, T. Stoeck, R. Ulber; Induction and Genetic Identification of a Callus-Like Growth Developed in The Brown Alga Fucus vesiculosus; Engineering in Life Sciences, https://doi.org/10.1002/elsc.201800211
Zayed, C. Dienemann, C. Giesec, R. Krämer, R. Ulber; An immobilized perylene diimide derivative for fucoidan purification from a crude brown algae extract; Process Biochemistry; https://www.sciencedirect.com/science/article/pii/S1359511317310711?via%3Dihub
A. Zayed, K. Muffler, T. Hahn, S. Rupp, D. Finkelmeier, A. Burger-Kentischer, R. Ulber; Physicochemical and biological characterization of fucoidan from Fucus vesiculosus purified by dye affinity chromatography; Marine Drugs (2016) DOI:10.3390/md14040079
A. Zayed, K. Muffler, R. Ulber; Novel and rapid extraction and purification procedure for fucoidan from F. vesiculosus; Jahrestreffen der Fachgruppe Phytoextrakte ProcessNET (2015) Bonn
A. Zayed, R. Ulber; Downstream Processing of Bioactive Heteropolysaccharides; ProcessNet-Jahrestagung und 32. DECHEMA-Jahrestagung der Biotechnologen (2016) Aachen
A. Zayed, B. Ledermann, N. Frankenberg-Dinkel, R. Ulber; Heterologous Expression of Heteropolysaccharides; ProcessNet-Jahrestagung und 32. DECHEMA-Jahrestagung der Biotechnologen (2016) Aachen
A. Zayed, K. Muffler, T. Hahn, R. Ulber; Cultivation of macro algae in wave bag reactors for the production of sulfated heteropolysaccharides; Himmelfahrtstagung: New Frontiers for Biotech-Processes (2016) Koblenz
A. Zayed, C. Dienemann, R. Krämer, R. Ulber. A Perylene Diimide Derivative for Sulphated Heteropolysaccharides Purification (Ex. Fucoidan from Crude Brown Algae Extract. French Chemical and Process Engineering Society (SFGP), (July 11-13th 2017, Nancy, France - German-French day - July 12th, 2017).
A. Zayed, C. Dienemann, R. Krämer, R. Ulber. A simple and Time-saving Purification of Fucoidan from Crude Brown Algae Extracts. Himmelfahrtstagung 2017: Models for Developing and Optimising Biotech Production, (May 22. - 24. 2017, Neu-Ulm, Germany).
A. Zayed, B. Ledermann, N. Frankenberg-Dinkel, R. Ulber. Evaluation of Heterologously Expressed Algal Fucosyltransferases and Sulfotransferases in E. coli. Himmelfahrtstagung 2017: Models for Developing and Optimising Biotech Production, (22. - 24. May 2017, Neu-Ulm, Germany).