Low-nutrient emersed fermentation of terrestrial cyanobacteria for the production of biotechnological materials

(German title: Nährstoffarme Emers-Fermentation terrestrischer Cyanobakterien zur Produktion biotechnischer Wertstoffe)

Terrestrial cyanobacteria are characterised by a high degree of adaptation to extreme locations such as hot and cold deserts. They can achieve comparatively high productivity in these locations despite limiting nutrient, light and water availability. Although this physiological efficiency indicates biotechnologically and economically valuable substances, these aerophilic organisms have not been used biotechnologically so far, as they are growth-limited under conventional submerged fermentation conditions.

The aim of this project is therefore to realise a new bioreactor concept in order to be able to cultivate these groups of organisms and test them for valuable substances in the future. The emerse aerosol-exposed production process offers advantages in terms of light and CO2 supply as well as nutrient allocation and controlled water supply for the cyanobacteria. In addition, by setting defined conditions, this fermentation method also enables targeted drying of the cyanobacteria, which can correlate with a change in quality or increase in quantity of certain valuable substances. Automated qualitative and quantitative production control is being sought during the project using non-invasive chlorophyll fluorescence technology, which is to be verified using stable isotope analyses.

Partner

Dr. Michael Lakatos (TU Kaiserslautern)

StatusCompleted project
Funding organisationGerman Research Foundation
Funding period03/2010-02/2012
Funding codeUL 170/7-1

Publications and conference papers

  • S. Kuhne, D. Strieth, M. Lakatos, K. Muffler, R. Ulber; A new photobioreactor concept enabling the production of desiccation induced biotechnological products using terrestrial cyanobacteria; Journal of Biotechnology 192 (2014) 28-33
  • K. Muffler, M. Lakatos, C. Schlegel, D. Strieth, S. Kuhne, R. Ulber; Application of Biofilm Bioreactors in White Biotechnology; Adv. Biochem. Eng. Biotechnol. 146 (2014) 123-161, DOI: 10.1007/10_2013_267
  • S. Kuhne, D. Strieth, A. Weber, K. Muffler, M. Lakatos, R. Ulber; Screening of two terrestrial cyanobacteria for biotechnological production processes in shaking flasks, bubble columns and stirred tank reactors; Journal of Applied Phycology 26 (2014) 1639-1648, DOI 10.1007/s10811-013-0224-z
  • S. Kuhne, M. Lakatos, S. Foltz, K. Muffler, R. Ulber; Characterisation of terrestrial cyanobacteria for establishing low energy consuming production processes of carotenoids; Sustainable Chemical Processes (2013) 1:6, DOI: 10.1186/2043-7129-1-6
  • K. Muffler, S. Kuhne, R. Ulber, M. Lakatos; Zugang zum Wertstoffspektrum terrestrischer Cyanobakterien GIT Labor-Fachzeitschrift 10 (2011) 670
  • S. Kuhne, K. Muffler, M.Lakatos, R. Ulber; Innovativer Photobioreaktor zur Kultivierung von terrestrischen Cyanobakterien; Jahrestagung ProcessNet 2012
  • K. Muffler, S. Kuhne, M. Lakatos, R. Ulber, Nutzung terrestrischer Cyanobakterien zur Wertstoffproduktion, 28. Jahrestagung der Biotechnologen (2010) Aachen
  • K. Muffler, M. Lakatos, R. Ulber, Utilization of terrestrial cyanobacteria for production of pharmaceutical compounds, 8th European Workshop Biotechnology of Microalgae (2010) Nuthetal
  • K. Muffler, M. Lakatos, R. Ulber, Biotechnologisches Verfahren zur nachhaltigen Gewinnung von Sauerstoff und Nahrungsmitteln aus humanen Stoffwechselendprodukten, Symposium Lunar Base + Bauen für ein Leben auf dem Mond (2009) Kaiserslautern