Publikation: Insight into Fucoidan-based PEGylated PLGA Nanoparticles Encapsulating Methyl Anthranilic Acid: In Vitro Evaluation and In Vivo Anti-inflammatory Study

Das Lehrgebiet BioVT publiziert mit Partnern der Tanta University (Ägypten) und der Princess Nourah bint Abdulrahman University (Saudi Arabien) in der Zeitschrift "Marine Drugs" den Artikel “Insight into Fucoidan-based PEGylated PLGA Nanoparticles Encapsulating Methyl Anthranilic Acid: In Vitro Evaluation and In Vivo Anti-inflammatory Study”.

A potential fucoidan-based PEGylated PLGA nanoparticles (NPs) offering a proper deliv-21 ery of N-methyl anthranilic acid (MA, a model of hydrophobic anti-inflammatory drug) have been 22 developed via the formation of fucoidan aqueous coating surrounding PEGylated PLGA NPs. The 23 optimum formulation (FuP2) composed of fucoidan:m-PEG-PLGA (1:0.5 w/w) with particle size 24 (365±20.76 nm), zeta potential (-22.30±2.56 mV), % entrapment efficiency (85.45±7.41), drug loading 25 (51.36±4.75 μg/mg of NPs), % initial burst (47.91±5.89), and % cumulative release (102.79±6.89) has 26 been furtherly investigated for the anti-inflammatory in vivo study. This effect of FuP2 was assessed 27 in rats' carrageenan-induced acute inflammation model. The average weight of the paw edema was 28 significantly lowered (p≤0.05) by treatment with FuP2. Moreover, cyclooxygenase-2 and tumor ne-29 crosis factor-alpha immunostaining were decreased in FuP2 treated group compared to the other 30 groups. The levels of prostaglandin E2, nitric oxide, and malondialdehyde were significantly re-31 duced (p≤0.05) in the FuP2-treated group. A significant reduction (p≤0.05) in the expression of inter-32 leukins (IL-1β and IL-6) with an improvement of the histological findings of the paw tissues was 33 observed in the FuP2-treated group. Thus, fucoidan-based PEGylated PLGA–MA NPs are a prom-34 ising anti-inflammatory delivery system that can be applied for other similar drugs potentiating 35 their pharmacological and pharmacokinetic properties.

D.H. Abdelkader, E. Elekhnawy, W.A. Negm, T.A. El-Masry, M. Almukainzi, A. Zayed, R. Ulber; Insight into Fucoidan-based PEGylated PLGA Nanoparticles Encapsulating Methyl Anthranilic Acid: In Vitro Evaluation and In Vivo Anti-inflammatory Study; Marine Drugs (2022) im Druck