Publikation: Fermentative terpenoid production from grass clippings

Das Lehrgebiet BioVT ist Co-Autor bei der Publikation "Fermentative terpenoid production from grass clippings", die in der Zeitschrift "Molecules" erscheint.

Green waste is currently insufficiently recycled and has untapped potential as a valuable resource. Our aim was to use juice from green waste as a growth medium for microorganisms. Herein, we demonstrate the production of the sesquiterpene α-humulene with the versatile organism Cupriavidus necator pKR-hum on a growth medium from grass clippings. The medium was compared with established media in terms of microbial growth and terpene production. C. necator pKR-hum shows a maximum growth rate of 0.43 h^-1 in the grass medium and 0.50 h^-1 in the LB medium. The grass medium was able to produce 2 mg/L of α-humulene compared to 10 mg/L in the LB medium. By concentrating the grass medium and using a controlled bioreactor in combination with an optimized in situ product removal, it is likely that comparable product concentrations could be achieved. To our best knowledge, this is the first time that juice from grass clippings has been used as a growth medium without any further additives for microbial product synthesis. This use of green waste as a material represents a new bio economic utilization option of waste materials and could contribute to improve the economics of grass biorefineries.

A. Langsdorf, A.-L. Drommershausen, M. Volkmar, R. Ulber, D. Holtmann; Fermentative terpenoid production from grass clippings; Molecules (2022) im Druck