Publikation: Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells

Das Lehrgebiet BioVT publiziert ein neues Verfahren zur Herstellung von Triterpenen mittels einer Basilikum-Zellkultur in Plant Cell, Tissue and Organ Culture.

The application of plant suspension culture to produce valuable compounds, such as triterpenoids oleanolic acid and ursolic acid, is a well-established alternative to the cultivation of whole plants. Cambial meristematic cells (CMCs) are a growing field of research, often showing superior cultivation properties compared to their dedifferentiated cell (DDC) counterparts. In this work, the first-time establishment of O. basilicum CMCs is demonstrated. DDCs and CMCs were cultivated in shake flasks and wave-mixed disposable bioreactors (wDBRs) and evaluated regarding triterpenoid productivity and biomass accumulation. CMCs showed characteristic small vacuoles and were found to be significantly smaller than DDCs. Productivities of oleanolic and ursolic acid of CMCs were determined at 3.02 ± 0.76 mg/(l*d) and 4.79 ± 0.48 mg/(l*d) after 19 d wDBR cultivation, respectively. These values were consistently higher than any productivities determined for DDCs over the observed cultivation period of 37 d. Elicitation with methyl jasmonate of DDCs and CMCs in shake flasks resulted in increased product contents up to 48 h after elicitor addition, with the highest increase found in CMCs at 232.30 ± 19.33 % (oleanolic acid) and 192.44 ± 18.23 % (ursolic acid) after 48 h.

A. Mehring, J. Haffelder, J. Chodorski, J. Stiefelmaier, D. Strieth, R. Ulber; Establishment and triterpenoid production of Ocimum basilicum cambial meristematic cells; Plant Cell, Tissue and Organ Culture (2020) im Druck